Medical Definition of NucleotideNucleotide: A subunit of DNA or RNA that consists of a nitrogenous base (A, G, T, or C in DNA; A, G, U, or C in RNA), a phosphate molecule, and a sugar molecule (deoxyribose in DNA, and ribose in RNA). Thousands of nucleotides are linked to form a DNA or an RNA molecule. Last Editorial Review: 3/19/2012
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