Definition of Hybridization, nucleic acid
Hybridization, nucleic acid: A laboratory technique in which single-stranded nucleic acids (DNA or RNA) are allowed to interact so that complexes called hybrids are formed by molecules with similar, complementary sequences.
Through nucleic acid hybridization, the degree of sequence identity between nucleic acids can be determined and specific sequences detected in them. The hybridization can be carried out in solution or with one component immobilized on a gel or, most commonly, on nitrocellulose paper.
Hybrids are detected by various means: visualization in the electron microscope; by radioactively labelling one component and removing non-complexed DNA; or by washing or digestion with an enzyme that attacks single-stranded nucleic acids and finally estimating the radioactivity bound.
Hybridizations are done in all combinations: DNA-DNA (DNA can be rendered single-stranded by heat denaturation), DNA-RNA or RNA-RNA.
In situ hybridization involves hybridizing a labelled nucleic acid (often labelled with a fluorescent dye) to suitably prepared cells or histological sections. This is used particularly to look for specific transcription or localization of genes to specific chromosomes via fluorescent in situ hybridization (FISH) analysis).
Last Editorial Review: 6/14/2012
Back to MedTerms online medical dictionary A-Z List
Need help identifying pills and medications?
- Allergic Skin Disorders
- Bacterial Skin Diseases
- Bites and Infestations
- Diseases of Pigment
- Fungal Skin Diseases
- Medical Anatomy and Illustrations
- Noncancerous, Precancerous & Cancerous Tumors
- Oral Health Conditions
- Papules, Scales, Plaques and Eruptions
- Scalp, Hair and Nails
- Sexually Transmitted Diseases (STDs)
- Vascular, Lymphatic and Systemic Conditions
- Viral Skin Diseases
- Additional Skin Conditions